Characterization of the endothelial cell cytoskeleton following HLA class I ligation.

ABSTRACT:

Background

Vascular endothelial cells (ECs) are a target of antibody-mediated allograft rejection. In vitro, when the HLA class I molecules on the surface of ECs are ligated by anti-HLA class I antibodies, cell proliferation and survival pathways are activated and this is thought to contribute to the development of antibody-mediated rejection. Crosslinking of HLA class I molecules by anti-HLA antibodies also triggers reorganization of the cytoskeleton, which induces the formation of F-actin stress fibers. HLA class I induced stress fiber formation is not well understood.

Methodology and principal findings

The present study examines the protein composition of the cytoskeleton fraction of ECs treated with HLA class I antibodies and compares it to other agonists known to induce

SUBMITTER: Ziegler ME 

PROVIDER: S-EPMC3256144 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature