Electrophoretic mobility shift assay reveals a novel recognition sequence for Setaria italica NAC protein.

ABSTRACT: The NAC (NAM/ATAF1,2/CUC2) proteins are among the largest family of plant transcription factors. Its members have been associated with diverse plant processes and intricately regulate the expression of several genes. Inspite of this immense progress, knowledge of their DNA-binding properties are still limited. In our recent publication,1 we reported isolation of a membrane-associated NAC domain protein from Setaria italica (SiNAC). Transactivation analysis revealed that it was a functionally active transcription factor as it could stimulate expression of reporter genes in vivo. Truncations of the transmembrane region of the protein lead to its nuclear localization. Here we describe expression and purification of SiNAC DNA-binding domain. We further report identification of a novel DNA-bind

SUBMITTER: Puranik S 

PROVIDER: S-EPMC3256391 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature