Xylan oligosaccharides and cellobiohydrolase I (TrCel7A) interaction and effect on activity.

ABSTRACT:

Background

The well-studied cellulase mixture secreted by Trichoderma reesei (anamorph to Hypocrea jecorina) contains two cellobiohydolases (CBHs), cellobiohydrolase I (TrCel7A) and cellobiohydrolase II (TrCeI6A), that are core enzymes for the solubilisation of cellulose. This has attracted significant research interest because of the role of the CBHs in the conversion of biomass to fermentable sugars. However, the CHBs are notoriously slow and susceptible to inhibition, which presents a challenge for the commercial utilisation of biomass. The xylans and xylan fragments that are also present in the biomass have been suggested repeatedly as one cause of the reduced activity of CHBs. Yet, the extent and mechanisms of this inhibition remain poorly elucidated. Therefore, we studied xyl

SUBMITTER: Baumann MJ 

PROVIDER: S-EPMC3257202 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature