Discovery of T cell antigens by high-throughput screening of synthetic minigene libraries.

ABSTRACT: The identification of novel T cell antigens is central to basic and translational research in autoimmunity, tumor immunology, transplant immunology, and vaccine design for infectious disease. However, current methods for T cell antigen discovery are low throughput, and fail to explore a wide range of potential antigen-receptor interactions. To overcome these limitations, we developed a method in which programmable microarrays are used to cost-effectively synthesize complex libraries of thousands of minigenes that collectively encode the content of hundreds of candidate protein targets. Minigene-derived mRNA are transfected into autologous antigen presenting cells and used to challenge complex populations of purified peripheral blood CD8+ T cells in multiplex, parallel ELISPOT assays. In th

SUBMITTER: Hondowicz BD 

PROVIDER: S-EPMC3257230 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature