Using engineered single-chain antibodies to correlate molecular binding properties and nanoparticle adhesion dynamics.

ABSTRACT: Elucidation of the relationship between targeting molecule binding properties and the adhesive behavior of therapeutic or diagnostic nanocarriers would aid in the design of optimized vectors and lead to improved efficacy. We measured the adhesion of 200-nm-diameter particles under fluid flow that was mediated by a diverse array of molecular interactions, including recombinant single-chain antibodies (scFvs), full antibodies, and the avidin/biotin interaction. Within the panel of scFvs, we used a family of mutants that display a spectrum of binding kinetics, allowing us to compare nanoparticle adhesion to bond chemistry. In addition, we explored the effect of molecular size by inserting a protein linker into the scFv fusion construct and by employing scFvs that are specific for targets with

SUBMITTER: Haun JB 

PROVIDER: S-EPMC3257898 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature