Genome-wide differential gene expression in immortalized DF-1 chicken embryo fibroblast cell line.

ABSTRACT:

Background

When compared to primary chicken embryo fibroblast (CEF) cells, the immortal DF-1 CEF line exhibits enhanced growth rates and susceptibility to oxidative stress. Although genes responsible for cell cycle regulation and antioxidant functions have been identified, the genome-wide transcription profile of immortal DF-1 CEF cells has not been previously reported. Global gene expression in primary CEF and DF-1 cells was performed using a 4X44K chicken oligo microarray.

Results

A total of 3876 differentially expressed genes were identified with a 2 fold level cutoff that included 1706 up-regulated and 2170 down-regulated genes in DF-1 cells. Network and functional analyses using Ingenuity Pathways Analysis (IPA, Ingenuity® Systems, http://www.ingenuity.com) revealed tha

SUBMITTER: Kong BW 

PROVIDER: S-EPMC3258366 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature