Climbing fiber activity reduces 14-3-3-θ regulated GABA(A) receptor phosphorylation in cerebellar Purkinje cells.

ABSTRACT: Cerebellar adaptive plasticity regulates posture and movement in response to changing conditions of sensory stimulation. Study of adaptive plasticity of cerebellar circuitry in vitro confines experimental interest to mechanisms with a time scale of minutes. However, cerebellar plasticity, measured behaviorally or electrophysiologically in vivo, occurs over a time scale of tens of minutes and hours. Here we investigate how optokinetically-evoked increases in climbing fiber activity influence expression of key subcellular signaling proteins that regulate the accumulation of GABA(A) receptors (GABA(A)Rs) in the cytoplasm of Purkinje cells and their insertion into the plasma membrane. We used long-term horizontal optokinetic stimulation (HOKS) to activate climbing fibers that project to the fl

SUBMITTER: Qian Z 

PROVIDER: S-EPMC3258370 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature