Cyclic AMP dysregulates intestinal epithelial cell restitution through PKA and RhoA.

ABSTRACT:

Background

Mucosal homeostasis is dependent on the establishment and maintenance of the cell-cell contacts that comprise the physiological barrier. Breaks in the barrier are linked to multiple diseases such as inflammatory bowel disease. While increased cyclic adenosine monophosphate (cAMP) levels limit inflammation by decreasing leukocyte infiltration, the effects of elevated cAMP on intestinal epithelial repair are unknown.

Methods

Restitution in animals administered rolipram was monitored by microscopic examination after laser wounding of the intestinal epithelium or in mice treated with dextran sodium sulfate (DSS). In vitro analysis was conducted using IEC6 and T84 cells to determine the role of elevated cAMP in altering Rho-dependent cellular migration signaling pathwa

SUBMITTER: Zimmerman NP 

PROVIDER: S-EPMC3258471 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature