Transcriptional profiling of innate and adaptive human immune responses to mycobacteria in the tuberculin skin test.

ABSTRACT: The tuberculin skin test (TST) is a model of integrated innate and adaptive human immune responses to Mycobacterium tuberculosis, but the component processes that are involved in this model have not previously been defined in vivo. We used transcriptional profiling to study these responses within the TST at molecular and system levels. Skin biopsies from TST injection sites were examined in subjects classified as TST(+) or TST(-) by clinical and histological criteria. Genome-wide expression arrays showed evolution of immune responses reflecting T-cell activation and recruitment with uniquely Th1-polarized responses and cytotoxic T cells (CTLs). In addition, distinct innate immune and IFN-γ-stimulated gene expression signatures were identified, under the regulation of NF-κB and STAT1 tran

SUBMITTER: Tomlinson GS 

PROVIDER: S-EPMC3258543 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature