Rapid detection of rpoB mutations in rifampin resistant M. tuberculosis from sputum samples by denaturing gradient gel electrophoresis.

ABSTRACT:

Objective

To establish a rapid detection method for identifying rpoB mutations associated with rifampin (RIF) resistance in sputum specimens.

Methods

We detected rpoB mutations directly in 90 sputum specimens collected from suspected tuberculosis patients using PCR-based denaturing gradient gel electrophoresis (DGGE) and compared these results with those obtained by rpoB sequencing and conventional drug susceptibility testing.

Results

The positive detection rate of Mycobacterium tuberculosis (M. tuberculosis) was 52.2% by Acid-Fast Bacilli staining and 72.2% by conventional mycobacterial culture. In contrast, the positive rate was significantly higher (93.3%) by PCR-based detection of the rpoB gene in the same specimens. Furthermore, 75% of the tested specimens presen

SUBMITTER: Li J 

PROVIDER: S-EPMC3258557 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature