High-level secretion of recombinant monomeric murine and human single-chain Fv antibodies from Drosophila S2 cells.

ABSTRACT: Single-chain variable fragment (scFvs) antibodies are small polypeptides (∼26 kD) containing the heavy (V(H)) and light (V(L)) immunoglobulin domains of a parent antibody connected by a flexible linker. In addition to being frequently used in diagnostics and therapy for an increasing number of human diseases, scFvs are important tools for structural biology as crystallization chaperones. Although scFvs can be expressed in many different organisms, the expression level of an scFv strongly depends on its particular amino acid sequence. We report here a system allowing for easy and efficient cloning of (i) scFvs selected by phage display and (ii) individual heavy and light chain sequences from hybridoma cDNA into expression plasmids engineered for secretion of the recombinant fragment produce

SUBMITTER: Gilmartin AA 

PROVIDER: S-EPMC3258843 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature