Ontology highlight
ABSTRACT:
SUBMITTER: Jasiak AJ
PROVIDER: S-EPMC3258907 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
The Journal of biological chemistry 20080730 39
Yeast RNA polymerase (Pol) II consists of a 10-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immunoprecipitation coupled to high resolution tiling microarray analysis. We show that the genome-wide occupancy profiles for Rpb7 and the core subunit Rpb3 are essentially identical. Thus, the complete Pol II associates with DNA in ...[more]