Tissue imaging using nanospray desorption electrospray ionization mass spectrometry.

ABSTRACT: Ambient ionization imaging mass spectrometry is uniquely suited for detailed spatially resolved chemical characterization of biological samples in their native environment. However, the spatial resolution attainable using existing approaches is limited by the ion transfer efficiency from the ionization region into the mass spectrometer. Here, we present a first study of ambient imaging of biological samples using nanospray desorption ionization (nano-DESI). Nano-DESI is a new ambient pressure ionization technique that uses minute amounts of solvent confined between two capillaries comprising the nano-DESI probe and the solid analyte for controlled desorption of molecules present on the substrate followed by ionization through self-aspirating nanospray. We demonstrate highly sensitive spati

SUBMITTER: Laskin J 

PROVIDER: S-EPMC3259225 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature