A Comparison of Hydroxyl Radical and Hydrogen Peroxide Generation in Ambient Particle Extracts and Laboratory Metal Solutions.

ABSTRACT: Generation of reactive oxygen species (ROS) - including superoxide ((•)O(2) (-)), hydrogen peroxide (HOOH), and hydroxyl radical ((•)OH) - has been suggested as one mechanism underlying the adverse health effects caused by ambient particulate matter (PM). In this study we compare HOOH and (•)OH production from fine and coarse PM collected at an urban (Fresno) and rural (Westside) site in the San Joaquin Valley (SJV) of California, as well as from laboratory solutions containing dissolved copper or iron. Samples were extracted in a cell-free, phosphate-buffered saline (PBS) solution containing 50 μM ascorbate (Asc). In our laboratory solutions we find that Cu is a potent source of both HOOH and (•)OH, with approximately 90% of the electrons that can be donated from Asc ending up in HOOH and

SUBMITTER: Shen H 

PROVIDER: S-EPMC3259706 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature