Recruitment of the RNA helicase RHAU to stress granules via a unique RNA-binding domain.

ABSTRACT: In response to environmental stress, the translation machinery of cells is reprogrammed. The majority of actively translated mRNAs are released from polysomes and driven to specific cytoplasmic foci called stress granules (SGs) where dynamic changes in protein-RNA interaction determine the subsequent fate of mRNAs. Here we show that the DEAH box RNA helicase RHAU is a novel SG-associated protein. Although RHAU protein was originally identified as an AU-rich element-associated protein involved in urokinase-type plasminogen activator mRNA decay, it was not clear whether RHAU could directly interact with RNA. We have demonstrated that RHAU physically interacts with RNA in vitro and in vivo through a newly identified N-terminal RNA-binding domain, which was found to be both essential and suffi

SUBMITTER: Chalupnikova K 

PROVIDER: S-EPMC3259895 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature