Rapid detection of wheat yellow mosaic virus by reverse transcription loop-mediated isothermal amplification.

ABSTRACT: For the detection of wheat yellow mosaic virus (WYMV), we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Using Primer Explorer software, four sets of primers were designed and RT-LAMP assay reaction conditions were optimized. The RT-LAMP was performed at different times by four primer sets. Agarose gel analysis showed that WYMV could be detected after 30 min with the primer set III and after 45 min with the other three primer sets, both under the 80-min reaction time. RT-LAMP had the same results with the four primer sets, thus primer set III and 65°C for 80 min reaction were selected for virus detection. There was no significant different when avian myeloblastosis virus (AMV) and moloney murine leukemia virus (M-MLV) RT-LAMP with the four prim

SUBMITTER: Zhang ZY 

PROVIDER: S-EPMC3260119 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature