Meta-analysis of oncogenic protein kinase Ciota signaling in lung adenocarcinoma.

ABSTRACT:

Purpose

Atypical protein kinase Ciota (PKCiota) is an oncogene in non-small cell lung cancer (NSCLC). Here, we identify four functional gene targets of PKCiota in lung adenocarcinoma (LAC), the most prominent form of NSCLC.

Experimental design

Three independent public domain gene expression data sets were interrogated to identify genes coordinately expressed with PKCiota in primary LAC tumors. Results were validated by QPCR in an independent set of primary LAC tumors. RNAi-mediated knockdown of PKCiota and the target genes was used to determine whether expression of the identified genes was regulated by PKCiota, and whether these target genes play a role in anchorage-independent growth and invasion of LAC cells.

Results

Meta-analysis identified seven genes whose expre

SUBMITTER: Erdogan E 

PROVIDER: S-EPMC3260476 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature