Simian virus 40 induces lamin A/C fluctuations and nuclear envelope deformation during cell entry.

ABSTRACT: The canonical gate of viruses and viral genomes into the nucleus in non-dividing cells is the nuclear pore, embedded within the nuclear envelope. However, we found that for SV40, the nuclear envelope poses a major hurdle to infection: FISH analysis revealed that the majority of viral DNA remains trapped in the ER; silencing of Lamin A/C rendered the cells more susceptible to infection; and proliferating cells are more susceptible to infection than quiescent cells. Surprisingly, we observed that following SV40 infection the nuclear envelope, including lamins A/C, B1, B2 and the nuclear pore complex, was dramatically deformed, as seen by immunohistochemistry. The infection induced fluctuations in the level of lamin A/C, dephosphorylation of an unknown epitope and leakage to the cytoplasm jus

SUBMITTER: Butin-Israeli V 

PROVIDER: S-EPMC3260569 | biostudies-literature | 2011 Jul-Aug

REPOSITORIES: biostudies-literature