Cryo-EM study of Hepatitis B virus core antigen capsids decorated with antibodies from a human patient.

ABSTRACT: The capsid (core antigen, HBcAg) is one of three major antigens present in patients infected with Hepatitis B virus. The capsids are icosahedral particles, whose most prominent features are spikes that extend 25 Å out from the contiguous "floor". At the spike tip are two copies of the "immunodominant loop". Previously, the epitopes of seven murine monoclonal antibodies have been identified by cryo-EM analysis of Fab-labeled capsids. All but one are conformational and all but one map around the spike tip. The exception, which is also the tightest-binder, straddles an inter-molecular interface on the floor. Seeking to relate these observations to the immunological response of infected humans, we isolated anti-cAg antibodies from a patient, prepared Fabs, and analyzed their binding to capsids

SUBMITTER: Kandiah E 

PROVIDER: S-EPMC3261347 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature