Nucleolar association and transcriptional inhibition through 5S rDNA in mammals.

ABSTRACT: Changes in the spatial positioning of genes within the mammalian nucleus have been associated with transcriptional differences and thus have been hypothesized as a mode of regulation. In particular, the localization of genes to the nuclear and nucleolar peripheries is associated with transcriptional repression. However, the mechanistic basis, including the pertinent cis- elements, for such associations remains largely unknown. Here, we provide evidence that demonstrates a 119 bp 5S rDNA can influence nucleolar association in mammals. We found that integration of transgenes with 5S rDNA significantly increases the association of the host region with the nucleolus, and their degree of association correlates strongly with repression of a linked reporter gene. We further show that this mechani

SUBMITTER: Fedoriw AM 

PROVIDER: S-EPMC3261910 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature