Prostaglandin E2 as an inhibitory modulator of fibrogenesis in human lung allografts.

ABSTRACT:

Rationale

Donor mesenchymal stromal/stem cell (MSC) expansion and fibrotic differentiation is associated with development of bronchiolitis obliterans syndrome (BOS) in human lung allografts. However, the regulators of fibrotic differentiation of these resident mesenchymal cells are not well understood.

Objectives

This study examines the role of endogenous and exogenous prostaglandin (PG)E2 as a modulator of fibrotic differentiation of human lung allograft-derived MSCs.

Methods

Effect of PGE2 on proliferation, collagen secretion, and α-smooth muscle actin (α-SMA) expression was assessed in lung-resident MSCs (LR-MSCs) derived from patients with and without BOS. The response pathway involved was elucidated by use of specific agonists and antagonists.

Measurement and

SUBMITTER: Walker NM 

PROVIDER: S-EPMC3262036 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature