Absence of integrin αvβ3 enhances vascular leak in mice by inhibiting endothelial cortical actin formation.

ABSTRACT:

Rationale

Sepsis and acute lung injury (ALI) have devastatingly high mortality rates. Both are associated with increased vascular leak, a process regulated by complex molecular mechanisms.

Objectives

We hypothesized that integrin αvβ3 could be an important determinant of vascular leak and endothelial permeability in sepsis and ALI.

Methods

β3 subunit knockout mice were tested for lung vascular leak after endotracheal LPS, and systemic vascular leak and mortality after intraperitoneal LPS and cecal ligation and puncture. Possible contributory effects of β3 deficiency in platelets and other hematopoietic cells were excluded by bone marrow reconstitution experiments. Endothelial cells treated with αvβ3 antibodies were evaluated for sphingosine-1 phosphate (S1P)–mediated

SUBMITTER: Su G 

PROVIDER: S-EPMC3262039 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature