Clinical implications and molecular mechanisms of immunoparalysis after cardiopulmonary bypass.

ABSTRACT:

Objective

We used a whole blood assay to characterize the immune system's response after cardiopulmonary bypass (CPB) in children to identify the risk for postoperative infections. We assessed the impact of CPB on histone methylation as a potential mechanism for altering gene expression necessary for the immune system's capacity to defend against infections.

Methods

We prospectively enrolled patients less than 18 years old undergoing heart surgery requiring CPB at C.S. Mott Children's Hospital. Blood was obtained from patients before CPB, on CPB, and on postoperative days 1, 3, and 5. Ex vivo lipopolysaccharide-induced tumor necrosis factor-alpha production measured the capacity of the immune system. Serum cytokines were measured using a multiplex assay. Chromatin immunoprec

SUBMITTER: Cornell TT 

PROVIDER: S-EPMC3262077 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature