Generation of tumor-initiating cells by exogenous delivery of OCT4 transcription factor.

ABSTRACT:

Introduction

Tumor-initiating cells (TIC) are being extensively studied for their role in tumor etiology, maintenance and resistance to treatment. The isolation of TICs has been limited by the scarcity of this population in the tissue of origin and because the molecular signatures that characterize these cells are not well understood. Herein, we describe the generation of TIC-like cell lines by ectopic expression of the OCT4 transcription factor (TF) in primary breast cell preparations.

Methods

OCT4 cDNA was over-expressed in four different primary human mammary epithelial (HMEC) breast cell preparations from reduction mammoplasty donors. OCT4-transduced breast cells (OTBCs) generated colonies (frequency ~0.01%) in self-renewal conditions (feeder cultures in human embryonic

SUBMITTER: Beltran AS 

PROVIDER: S-EPMC3262206 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature