HopX1 in Erwinia amylovora functions as an avirulence protein in apple and is regulated by HrpL.

ABSTRACT: Fire blight is a devastating disease of rosaceous plants caused by the Gram-negative bacterium Erwinia amylovora. This pathogen delivers virulence proteins into host cells utilizing the type III secretion system (T3SS). Expression of the T3SS and of translocated and secreted substrates is activated by the alternative sigma factor HrpL, which recognizes hrp box promoters upstream of regulated genes. A collection of hidden Markov model (HMM) profiles was used to identify putative hrp boxes in the genome sequence of Ea273, a highly virulent strain of E. amylovora. Among potential virulence factors preceded by putative hrp boxes, two genes previously known as Eop3 and Eop2 were characterized. The presence of functionally active hrp boxes upstream of these two genes was confirmed by β-glucuroni

SUBMITTER: Bocsanczy AM 

PROVIDER: S-EPMC3264070 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature