More than just a metabolic regulator--elucidation and validation of new targets of PdhR in Escherichia coli.

ABSTRACT:

Background

The pyruvate dehydrogenase regulator protein (PdhR) of Escherichia coli acts as a transcriptional regulator in a pyruvate dependent manner to control central metabolic fluxes. However, the complete PdhR regulon has not yet been uncovered. To achieve an extended understanding of its gene regulatory network, we combined large-scale network inference and experimental verification of results obtained by a systems biology approach.

Results

22 new genes contained in two operons controlled by PdhR (previously only 20 regulatory targets in eight operons were known) were identified by analysing a large-scale dataset of E. coli from the Many Microbes Microarray Database and novel expression data from a pdhR knockout strain, as well as a PdhR overproducing strain. We identif

SUBMITTER: Gohler AK 

PROVIDER: S-EPMC3265435 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature