Density of functional Ca2+ release-activated Ca2+ (CRAC) channels declines after T-cell activation.

ABSTRACT: CRAC channel-mediated Ca(2+) entry plays a crucial role in T lymphocyte activation. Activated T cells display enhanced Ca(2+) signaling compared with resting T cells; this is partially attributed to activation-induced upregulation of CRAC channel expression. Orai and Stim family genes encode CRAC channel structural elements and regulatory proteins, respectively, but studies of their expression in T cells have led to controversial results. We re-examined Orai and Stim gene expression in resting, activated, and Jurkat T cells. Levels of Orai1 transcripts, encoding the human T cell CRAC channel subunit, were not significantly different between resting T and activated T cells. The total amount of all Orai transcripts was 2-fold higher in activated T cells than in resting T cells. Orai1 and tot

SUBMITTER: Thakur P 

PROVIDER: S-EPMC3265799 | biostudies-literature | 2011 Nov-Dec

REPOSITORIES: biostudies-literature