Dictyostelium discoideum developmentally regulated genes whose expression is dependent on MADS box transcription factor SrfA.

ABSTRACT: The MADS box transcription factor SrfA is required for spore differentiation in Dictyostelium discoideum. srfA null strains form rounded spores that do not resist adverse environmental conditions. Five genes whose expression is dependent on SrfA have been isolated by differential hybridization. One of these genes, sigC, is identical to phg1b, previously characterized in mutants with altered adhesive properties and found to encode a nine-transmembrane-domain protein. This gene is transcribed into two mRNAs as the result of alternative splicing of two internal exons. The slower-migrating mRNA codes for a shorter protein that lacks the first transmembrane fragment and is not expressed in srfA null strains. The other four genes (sigA, sigB, sigD, and 45D) are expressed only during late develop

SUBMITTER: Escalante R 

PROVIDER: S-EPMC326651 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature