Directional gene expression and antisense transcripts in sexual and asexual stages of Plasmodium falciparum.

ABSTRACT:

Background

It has been shown that nearly a quarter of the initial predicted gene models in the Plasmodium falciparum genome contain errors. Although there have been efforts to obtain complete cDNA sequences to correct the errors, the coverage of cDNA sequences on the predicted genes is still incomplete, and many gene models for those expressed in sexual or mosquito stages have not been validated. Antisense transcripts have widely been reported in P. falciparum; however, the extent and pattern of antisense transcripts in different developmental stages remain largely unknown.

Results

We have sequenced seven bidirectional libraries from ring, early and late trophozoite, schizont, gametocyte II, gametocyte V, and ookinete, and four strand-specific libraries from late trophozoite

SUBMITTER: Lopez-Barragan MJ 

PROVIDER: S-EPMC3266614 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature