Diblock copolymers with tunable pH transitions for gene delivery.

ABSTRACT: A series of diblock copolymers containing an endosomal-releasing segment composed of diethylaminoethyl methacrylate (DEAEMA) and butyl methacrylate (BMA) were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. The materials were designed to condense plasmid DNA (pDNA) through electrostatic interactions with a cationic poly(N,N-dimethylaminoethyl methacrylate) (DMAEMA) first block. The pDMAEMA was employed as a macro chain transfer agent (macroCTA) for the synthesis of a series in which the relative feed ratios of DEAEMA and BMA were systematically varied from 20% to 70% BMA. The resultant diblock copolymers exhibited low polydispersity (PDI ≤ 1.06) with similar molecular weights (M(n) = 19.3-23.1 kDa). Dynamic light scattering (DLS) measurements in comb

SUBMITTER: Manganiello MJ 

PROVIDER: S-EPMC3266685 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature