Assessing genetic polymorphisms using DNA extracted from cells present in saliva samples.

ABSTRACT:

Background

Technical advances following the Human Genome Project revealed that high-quality and -quantity DNA may be obtained from whole saliva samples. However, usability of previously collected samples and the effects of environmental conditions on the samples during collection have not been assessed in detail. In five studies we document the effects of sample volume, handling and storage conditions, type of collection device, and oral sampling location, on quantity, quality, and genetic assessment of DNA extracted from cells present in saliva.

Methods

Saliva samples were collected from ten adults in each study. Saliva volumes from .10-1.0 ml, different saliva collection devices, sampling locations in the mouth, room temperature storage, and multiple freeze-thaw cycles wer

SUBMITTER: Nemoda Z 

PROVIDER: S-EPMC3267685 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature