Effect of Cu supplementation on genomic instability in chemically-induced mammary carcinogenesis in the rat.

ABSTRACT:

Background

The aim of the present study was to assess the effect of dietary supplementation (copper or copper and resveratrol) on the intensity of carcinogenesis and the frequency of microsatellite instability in a widely used model of mammary carcinogenesis induced in the rat by treatment with 7,12-dimethylbenz[a]anthracene (DMBA).

Methods

DNA was extracted from rat mammary cancers and normal tisues, amplified by PCR, using different polymorphic DNA markers and the reaction products were analyzed for microsatellite instability.

Results

It was found that irrespectively of the applied diet there was no inhibition of mammary carcinogenesis in the rats due to DMBA. Besides, in the groups supplemented with Cu (II) or Cu (II) and resveratrol the tumor formation was clearly

SUBMITTER: Bobrowska B 

PROVIDER: S-EPMC3267691 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature