A novel mean-centering method for normalizing microRNA expression from high-throughput RT-qPCR data.

ABSTRACT:

Background

Normalization is critical for accurate gene expression analysis. A significant challenge in the quantitation of gene expression from biofluids samples is the inability to quantify RNA concentration prior to analysis, underscoring the need for robust normalization tools for this sample type. In this investigation, we evaluated various methods of normalization to determine the optimal approach for quantifying microRNA (miRNA) expression from biofluids and tissue samples when using the TaqMan® Megaplex™ high-throughput RT-qPCR platform with low RNA inputs.

Findings

We compared seven normalization methods in the analysis of variation of miRNA expression from biofluid and tissue samples. We developed a novel variant of the common mean-centering normalization strategy,

SUBMITTER: Wylie D 

PROVIDER: S-EPMC3267743 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature