Time-lapse imaging of the dynamics of CNS glial-axonal interactions in vitro and ex vivo.

ABSTRACT:

Background

Myelination is an exquisite and dynamic example of heterologous cell-cell interaction, which consists of the concentric wrapping of multiple layers of oligodendrocyte membrane around neuronal axons. Understanding the mechanism by which oligodendrocytes ensheath axons may bring us closer to designing strategies to promote remyelination in demyelinating diseases. The main aim of this study was to follow glial-axonal interactions over time both in vitro and ex vivo to visualize the various stages of myelination.

Methodology/principal findings

We took two approaches to follow myelination over time: i) time-lapse imaging of mixed CNS myelinating cultures generated from mouse spinal cord to which exogenous GFP-labelled murine cells were added, and ii) ex vivo imaging of

SUBMITTER: Ioannidou K 

PROVIDER: S-EPMC3267745 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature