Severe defects in absorptive ion transport in distal colons of mice that lack ClC-2 channels.

ABSTRACT:

Background & aims

The fluid secretion model predicts that intestinal obstruction disorders can be alleviated by promoting epithelial Cl(-) secretion. The adenosine 3',5'-cyclic monophosphate (cAMP)-activated anion channel CFTR mediates Cl(-)-dependent fluid secretion in the intestine. Although the role of the ClC-2 channel has not been determined in the intestine, this voltage-gated Cl(-) channel might compensate for the secretory defects observed in patients with cystic fibrosis and other chronic constipation disorders. We investigated whether mice that lack ClC-2 channels (Clcn2(-/-)) have defects in intestinal ion transport.

Methods

Immunolocalization and immunoblot analyses were used to determine the cellular localization and the amount of ClC-2 expressed in mouse early

SUBMITTER: Catalan MA 

PROVIDER: S-EPMC3267842 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature