Biomimetic extracellular matrix-incorporated scaffold induces osteogenic gene expression in human marrow stromal cells.

ABSTRACT: Engineering biomaterials mimicking the biofunctionality of the extracellular matrix (ECM) is important in instructing and eliciting cell response. The native ECM is highly dynamic and has been shown to support cellular attachment, migration, and differentiation. The advantage of synthesizing an ECM-based biomaterial is that it mimics the native cellular environment. However, the ECM has tissue-specific composition and patterned arrangement. In this study, we have employed biomimetic strategies to develop a novel collagen/chitosan template that is embedded with the native ECM of differentiating human marrow stromal cells (HMSCs) to facilitate osteoblast differentiation. The scaffold was characterized for substrate stiffness by magnetic resonance imaging and nanoindentation and by immunohist

SUBMITTER: Ravindran S 

PROVIDER: S-EPMC3267968 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature