Refinement of Bos taurus sequence assembly based on BAC-FISH experiments.

ABSTRACT:

Background

The sequencing of the cow genome was recently published (Btau_4.0 assembly). A second, alternate cow genome assembly (UMD2), based on the same raw sequence data, was also published. The two assemblies have been subsequently updated to Btau_4.2 and UMD3.1, respectively.

Results

We compared the Btau_4.2 and UMD3.1 alternate assemblies. Inconsistencies were grouped into three main categories: (i) DNA segments showing almost coincidental chromosomal mapping but discordant orientation (inversions); (ii) DNA segments showing a discordant map position along the same chromosome; and (iii) sequences present in one chromosomal assembly but absent in the corresponding chromosome of the other assembly. The latter category mainly consisted of large amounts of scaffolds that we

SUBMITTER: Partipilo G 

PROVIDER: S-EPMC3268123 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature