Detection of microRNAs in color space.

ABSTRACT:

Motivation

Deep sequencing provides inexpensive opportunities to characterize the transcriptional diversity of known genomes. The AB SOLiD technology generates millions of short sequencing reads in color-space; that is, the raw data is a sequence of colors, where each color represents 2 nt and each nucleotide is represented by two consecutive colors. This strategy is purported to have several advantages, including increased ability to distinguish sequencing errors from polymorphisms. Several programs have been developed to map short reads to genomes in color space. However, a number of previously unexplored technical issues arise when using SOLiD technology to characterize microRNAs.

Results

Here we explore these technical difficulties. First, since the sequenced reads are l

SUBMITTER: Marco A 

PROVIDER: S-EPMC3268249 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature