CpG island methylator phenotype associates with low-degree chromosomal abnormalities in colorectal cancer.

ABSTRACT:

Purpose

Aberrant promoter methylation and genomic instability occur frequently during colorectal cancer development. CpG island methylator phenotype (CIMP) has been shown to associate with microsatellite instability, and BRAF mutation and is often found in the right-side colon. Nevertheless, the relative importance of CIMP and chromosomal instability (CIN) for tumorigenesis has yet to be thoroughly investigated in sporadic colorectal cancers.

Experimental design

We determined CIMP in 161 primary colorectal cancers and 66 matched normal mucosae using a quantitative bisulfite/PCR/ligase detection reaction (LDR)/Universal Array assay. The validity of CIMP was confirmed in a subset of 60 primary tumors using MethyLight assay and five independent markers. In parallel, CIN was ana

SUBMITTER: Cheng YW 

PROVIDER: S-EPMC3268558 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature