Genetic and physical interaction of the B-cell systemic lupus erythematosus-associated genes BANK1 and BLK.

ABSTRACT:

Objectives

Altered signalling in B cells is a predominant feature of systemic lupus erythematosus (SLE). The genes BANK1 and BLK were recently described as associated with SLE. BANK1 codes for a B-cell-specific cytoplasmic protein involved in B-cell receptor signalling and BLK codes for an Src tyrosine kinase with important roles in B-cell development. To characterise the role of BANK1 and BLK in SLE, a genetic interaction analysis was performed hypothesising that genetic interactions could reveal functional pathways relevant to disease pathogenesis.

Methods

The GPAT16 method was used to analyse the gene-gene interactions of BANK1 and BLK. Confocal microscopy was used to investigate co-localisation, and immunoprecipitation was used to verify the physical interaction of BANK1

SUBMITTER: Castillejo-Lopez C 

PROVIDER: S-EPMC3268679 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature