HEV-Capsid Protein Interacts With Cytochrome P4502C8 and Retinol-Binding Protein 4.

ABSTRACT:

Background

Hepatitis E virus (HEV) is a major causative agent of acute clinical hepatitis in adults throughout much of Asia, the Middle East, and Africa. The lack of an efficient cell culture system for HEV has greatly limited our understanding of the mechanisms of infection, replication, and pathogenicity of this virus. The yeast two-hybridization system is considered to be an efficient method for determining protein-protein interactions and screening interactive proteins associated with host cells.

Objectives

In order to identify the host-cell proteins interacting with the HEV-capsid proteins, a fragment of the HEV-capsid protein p239 (amino acids 368-606) was used as bait; human liver cDNA library was used as a source of host-cell proteins, and the screening was performed

SUBMITTER: Shen Q 

PROVIDER: S-EPMC3269060 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature