Molecular structure and peptidoglycan recognition of Mycobacterium tuberculosis ArfA (Rv0899).

ABSTRACT: Mycobacterium tuberculosis ArfA (Rv0899) is a membrane protein encoded by an operon that is required for supporting bacterial growth in acidic environments. Its C-terminal domain (C domain) shares significant sequence homology with the OmpA-like family of peptidoglycan-binding domains, suggesting that its physiological function in acid stress protection may be related to its interaction with the mycobacterial cell wall. Previously, we showed that ArfA forms three independently structured modules, and we reported the structure of its central domain (B domain). Here, we describe the high-resolution structure and dynamics of the C domain, we identify ArfA as a peptidoglycan-binding protein and we elucidate the molecular basis for its specific recognition of diaminopimelate-type peptidoglycan.

SUBMITTER: Yao Y 

PROVIDER: S-EPMC3269530 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature