Development of NMDA R1 expression in chicken auditory brainstem.

ABSTRACT: NMDA receptor subunit 1 (NR1) expression in the chicken cochlear nuclei was examined using immunohistochemistry and quantitative Western blots. An antibody raised in mouse against a highly conserved domain of NR1 recognized the same 115 kDa protein band in chicken brain. Quantitative Western blotting of cochlear nucleus protein showed no significant change in NR1 expression from E18 to adult. The nucleus angularis (NA) initiated NR1 expression before E12 that became more prominent after hatching. NR1-ir first appeared in the nucleus magnocellularis (NM) and nucleus laminaris (NL) at E10. From E12 to E19, NM exhibited a gradient in NR1 expression with medial, higher best frequency cell bodies being more immunoreactive than lateral, lower best frequency cell bodies. This gradient disappeared

SUBMITTER: Tang YZ 

PROVIDER: S-EPMC3269632 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature