Proteomic analysis of endothelial cold-adaptation.

ABSTRACT:

Background

Understanding how human cells in tissue culture adapt to hypothermia may aid in developing new clinical procedures for improved ischemic and hypothermic protection. Human coronary artery endothelial cells grown to confluence at 37°C and then transferred to 25°C become resistant over time to oxidative stress and injury induced by 0°C storage and rewarming. This protection correlates with an increase in intracellular glutathione at 25°C. To help understand the molecular basis of endothelial cold-adaptation, isolated proteins from cold-adapted (25°C/72 h) and pre-adapted cells were analyzed by quantitative proteomic methods and differentially expressed proteins were categorized using the DAVID Bioinformatics Resource.

Results

Cells adapted to 25°C expressed changes i

SUBMITTER: Zieger MA 

PROVIDER: S-EPMC3270058 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature