Safety assessment of Bifidobacterium longum JDM301 based on complete genome sequences.

ABSTRACT:

Aim

To assess the safety of Bifidobacterium longum (B. longum) JDM301 based on complete genome sequences.

Methods

The complete genome sequences of JDM301 were determined using the GS 20 system. Putative virulence factors, putative antibiotic resistance genes and genes encoding enzymes responsible for harmful metabolites were identified by blast with virulence factors database, antibiotic resistance genes database and genes associated with harmful metabolites in previous reports. Minimum inhibitory concentration of 16 common antimicrobial agents was evaluated by E-test.

Results

JDM301 was shown to contain 36 genes associated with antibiotic resistance, 5 enzymes related to harmful metabolites and 162 nonspecific virulence factors mainly associated with transcriptional

SUBMITTER: Wei YX 

PROVIDER: S-EPMC3270512 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature