Expression profiling and comparative genomics identify a conserved regulatory region controlling midline expression in the zebrafish embryo.

ABSTRACT: Differential gene transcription is a fundamental regulatory mechanism of biological systems during development, body homeostasis, and disease. Comparative genomics is believed to be a rapid means for the identification of regulatory sequences in genomes. We tested this approach to identify regulatory sequences that control expression in the midline of the zebrafish embryo. We first isolated a set of genes that are coexpressed in the midline of the zebrafish embryo during somitogenesis stages by gene array analysis and subsequent rescreens by in situ hybridization. We subjected 45 of these genes to Compare and DotPlot analysis to detect conserved sequences in noncoding regions of orthologous loci in the zebrafish and Takifugu genomes. The regions of homology that were scored in nonconserved

SUBMITTER: Dickmeis T 

PROVIDER: S-EPMC327098 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature