Remobilization of Sleeping Beauty transposons in the germline of Xenopus tropicalis.

ABSTRACT:

Background

The Sleeping Beauty (SB) transposon system has been used for germline transgenesis of the diploid frog, Xenopus tropicalis. Injecting one-cell embryos with plasmid DNA harboring an SB transposon substrate together with mRNA encoding the SB transposase enzyme resulted in non-canonical integration of small-order concatemers of the transposon. Here, we demonstrate that SB transposons stably integrated into the frog genome are effective substrates for remobilization.

Results

Transgenic frogs that express the SB10 transposase were bred with SB transposon-harboring animals to yield double-transgenic 'hopper' frogs. Remobilization events were observed in the progeny of the hopper frogs and were verified by Southern blot analysis and cloning of the novel integrations site

SUBMITTER: Yergeau DA 

PROVIDER: S-EPMC3271037 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature