Role of insulin-like growth factor-1 signaling pathway in cisplatin-resistant lung cancer cells.

ABSTRACT:

Purpose

The development of drug-resistant phenotypes has been a major obstacle to cisplatin use in non-small-cell lung cancer. We aimed to identify some of the molecular mechanisms that underlie cisplatin resistance using microarray expression analysis.

Methods and materials

H460 cells were treated with cisplatin. The differences between cisplatin-resistant lung cancer cells and parental H460 cells were studied using Western blot, MTS, and clonogenic assays, in vivo tumor implantation, and microarray analysis. The cisplatin-R cells were treated with human recombinant insulin-like growth factor (IGF) binding protein-3 and siRNA targeting IGF-1 receptor.

Results

Cisplatin-R cells illustrated greater expression of the markers CD133 and aldehyde dehydrogenase, more rapid

SUBMITTER: Sun Y 

PROVIDER: S-EPMC3271860 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature