Nonrandom HIV-1 infection and double infection via direct and cell-mediated pathways.

ABSTRACT: Cells infected with two related retroviruses can generate heterozygous virions, which are the precursors of recombinant proviruses. Although many studies have focused on the frequencies and mechanisms of retroviral recombination, little is known about the dynamics of double infection. To examine this issue, viruses generated from two HIV-1 vectors containing different markers were mixed together, and were used to infect target cells. The numbers of cells expressing none, one, or both markers were measured and were used to calculate whether double infection occurred at frequencies expected from random infection events. We found that double infection occurred significantly more frequently than predicted from random distribution; increased rates of double infection were observed in both a T c

SUBMITTER: Dang Q 

PROVIDER: S-EPMC327199 | biostudies-literature | 2004 Jan

REPOSITORIES: biostudies-literature