Integrative proteomic analysis of the nucleus accumbens in rhesus monkeys following cocaine self-administration.

ABSTRACT: The reinforcing effects and long-term consequences of cocaine self-administration have been associated with brain regions of the mesolimbic dopamine pathway, namely the nucleus accumbens (NAc). Studies of cocaine-induced biochemical adaptations in rodent models have advanced our knowledge; however, unbiased detailed assessments of intracellular alterations in the primate brain are scarce, yet essential, to develop a comprehensive understanding of cocaine addiction. To this end, two-dimensional difference in gel electrophoresis (2D-DIGE) was used to compare changes in cytosolic protein abundance in the NAc between rhesus monkeys self-administering cocaine and controls. Following image normalization, spots with significantly differential image intensities (P<0.05) were identified, excised, t

SUBMITTER: Tannu NS 

PROVIDER: S-EPMC3272768 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature